VOGELBUSCH BIOPHARMA is your competent partner for the design and supply of process facilities for product preparation and purification. We provide consultation, planning and procurement services according to each customer’s wishes, from single units to entire downstream lines including buffer supply:

indispensable for downstream processing  

• Buffer preparation tanks 
• Solid matter feeding systems (e.g. big-bag handling); also featuring dust extraction systems to prevent contamination 
• Buffer filtration (e.g. bioburden reduction using 0.2 µm filters) 
• •Filling stations for buffer storage tanks or bags 

used by many processes as an important purification stage

• Inline dilution, gradient systems, chromatography 
• Fractionation in mobile tanks or bags 
• Chromatography columns 
• Packing concepts as well as equipment for chromatography columns 

Chromatography systems are among the most complex functional units in modern biotechnology. Chromatography skids by VOGELBUSCH BIOPHARMA are mobile and flexible solutions for any type of chromatography process.

Typical features of chromatography skids

• Inline dilution of buffers and products and independent pumps for gradient feed mixing
• Typically up to 10 process inlets and 5 process outlets 
• Sampling points before and after the chromatography column connections 
• Mass flow control of flow rates by means of a flow meter mounted after every pump 
• Double tube heat exchangers ensure a constant temperature in the product flow before the Chromatography column   
• Bubble trap and gas sensors prevent gas bubbles from entering the chromatography column 
• Inline filtration can be used to reduce the bioburden
• Pressure, temperature, conductivity, pH, UV and flow rates are recorded before and after the chromatography column 
• Elution can be controlled by pH, UV, conductivity, volume or duration 
• Pressure can be controlled for chromatography columns with low operating pressures  
• Compatible with all standard process control systems  

Possible applications

There is at least one chromatography step used for downstream purification in todays biopharmaceutical manufacturing processes. These custom made chromatography skids, executed by VOGELBUSCH BIOPHARMA, are used in a variety of processes worldwide. Typical applications is the production of supplements, vaccines and medication.

Typical features of filtration skids

• Modular construction suitable for various liquid filtration applications
• Flexible construction enables applications with single or multiple filter arrays and different filter configurations
• Filter lines with
  • Pre- and main filter-units for bacterial count reduction
  • Simple adaption mechanisms enable the use of a variety of filter housings (single – or multi-element housing with variable heights)
  • Skids and all components are suitable for CIP (cleaning in place) and SIP (sterilization/sanitization in place)
  • Optimized skid structure
    • Rinse- and product-recovery steps (connections for buffer and high-purity water)
    • Separate SIP for filter groups (pre-filter and main-filter)
    • Filter integrity test of membrane filters
    • Monitoring of inlet- and differential-pressure
    • Monitoring and controlling of flow rate and volume
    • Compatible with all common process control systems (PCS)

Liquid filtration-steps are standard methods of modern biotechnology.

Possible applications

The various liquid filtration methods are used in a wide variety of applications in the modern biopharmaceutical industry.
Due to the process-specific development of each individual VOGELBUSCH BIOPHARMA filtration skid, they are used in various pharmaceutical plants. Some main applications are the production of antibodies, enzymes and hormones.

• Particle separation and baterial count reduction with membrane filter elements after media-/ buffer preparation
• Purification of fermentation solutions or crude protein solutions by means of depth filtration (disk stack modules), cassette filters or membrane filters at the start of the downstream process
• Purification of intermediates by depth filtration
• Bacterial count reduction with membrane filter elements for intermediate and final products
• Nanofiltration for virus removal

Typical features of ultrafiltration skids

• Circulation/product tanks optimized for minimum working volumes of up to 5 % of net volume
• Use of rotary lobe pumps in special arrangement to avoid shear forces and in hygienic design with double-sealing mechanical seals
• Modular design for the use of single or multiple cassette holders
• Suitable for all common types of cassettes
• Mechanically or hydraulically closing cassette holders
• Optimized prefabrication (preassembling) to minimize dead volumes
• Cleaning tank for the preparation and storage of cleaning solutions
• All tanks and vessels used can be heated and cooled depending on the intended use
• Double-tube heat exchangers can be used for temperature control of the retentate
• All systems are optimized for CIP (cleaning in place) and SIP (sterilization/sanitization in place – except cassette holder)
• Flow rate control by transmembrane pressure (TMP)
• Monitoring of temperature and flow rate on the retentate side
• Monitoring of UV and flow rate on the permeate side (optionally of pH and conductivity)
• Compatibility with all common process control systems (PCS)
• The use of other membrane systems (spiral wound, hollow-fibres) is possible on request

Possible applications

Todays most biopharmaceutical proceses utilize ultra- and diafiltration processes in downstream purification or end product processing. Due to the process-specific design of VOGELBUSCH BIOPHARMA’s ultrafiltration skids, they are used in a wide variety of processes worldwide. Some of the most important applications are the production of enzymes, hormones and antibodies.

Equipped for a large number of different tasks

• PEGylation
• Reduction reactions
• Crystallization 
• Mobile tanks and small containers for chemicals

• Sterile filtration
• Filling concepts, laminar flow workbenches
• Deep freezing systems