Fermentation & Harvest

Vogelbusch designs and delivers process plants for the upstream process

• Media preparation
  batch tanks for media preparation, with attached sterilization or media filtration (deep bed filtration, bioburden reduction with 0.2 µm filter)
• Media storage and filling (bags)
• Fermentation
  Bioreactors for microbial fermentation, suspension culture of mammalian cells, culture of adherent animal cells on microcarriers.
  Bioreactor technologies:
  • Microbial fermentation - batch, fed batch
  • Cell culture - batch, fed batch, continuously with perfusion (recovery of cell-free culture supernatants), dialysis fermenters (Removal of growth/product formation-inhibiting by products from the fermentation medium)
• Harvest
  Cell separation by microfiltration or other separation techniques for recovery of biomass or the cell-free culture supernatant. High-pressure homogenizers for cell disruption (processes with microbial fermentation

Prefabricated plants:

• Pilot plants for clinical batch production
• Multiline fermentation plants with growing, preculturing, and production bioreactors

Areas of application:

Microbial fermentation: production of active pharmaceutical ingredients with recombinant microorganisms, production of enzymes and hormones

Cell culture: production of active pharmaceutical ingredients (enzymes and hormones), production of monoclonal antibodies for therapeutic and diagnostic applications, production of virus vaccines.